Fig. 3: CrebA and CrebB knock downs do not affect period-luciferase oscillations in central clock neurons.
From: Reassessing the involvement of the CREB pathway in the circadian clock of Drosophila melanogaster
LABL analysis of flies expressing RNAi constructs for CrebA and CrebB in all clock neurons. Male flies with the genotypes w; Clk856-Gal4/LABL; UAS-CrebA-RNAi3/UAS-FLP, w; Clk856-Gal4/LABL; UAS-CrebB-RNAi2/UAS-FLP, and w; Clk856-Gal4/LABL; UAS-FLP/+ as control, were measured in a LumiCycle luminometer (Actimetrics) for 7-8 consecutive days in DD at 25 °C. a Bioluminescence oscillations of LABL flies expressing per-luc in all clock neurons plotted over time. Oscillations of CrebA-RNAi (top, blue) and CrebB-RNAi (bottom, red) flies from one representative experiment are plotted over time in comparison to the same control (black). Light grey shading indicates subjective day, dark grey subjective night, respectively.b Average period of per-luc oscillations in clock neurons Colored dots indicate period values of significant curve fits between 16 and 36 h incorporating data from all experimental repeats (n = 3 for CrebA-RNAi and CrebB-RNAi, n = 4 for control). c period changes of per-luc oscillations calculated by Morlet-wavelet-fitting over time (Material and methods39). Genotypes same as in A and B. Colored dots indicate significant periods as in B, with different colors representing independent experimental repeats. Periods with confidence intervals of 25% or less were omitted.
