Fig. 4: CrebB and CrebA knockout flies show normal response to brief light-pulses.
From: Reassessing the involvement of the CREB pathway in the circadian clock of Drosophila melanogaster
Phase delays and phase advances of indicated genotypes after a brief light pulse either at ZT15 or at ZT21, compared to non-pulsed (NP) controls, visualized using estimation statistics. Top: swarm plots of all individual phases measured on the first day of DD; bottom: distribution of bootstrapped-resampled mean phase differences between non-pulsed and light-pulsed control (left) and experimental (middle) flies (primary deltas); right: distribution of the mean difference between the primary deltas (delta-delta), revealing no phase shift differences between control and experimental flies after light pulses at ZT15 and ZT21. In the effect size half-violin plot, black dot indicates mean of the distribution, and black vertical bars indicate 95% confidence intervals. 5000 bootstrap samples were taken; confidence intervals are bias-corrected and accelerated. p values report results of two-sided permutation t-test. CrebB: For ZT15, the unpaired mean differences are −2.92 h between ZT15 control and NP control (p = 0.0) and −2.82 h between ZT15 knockout and NP knockout (p = 0.0). The delta-delta between control and knockout is 0.10 h (p = 0.909). For ZT21, the unpaired mean differences are 1.64 h between ZT21 control and NP control (p = 0.0) and 1.61 h between ZT21 knockout and NP knockout (p = 0.0002). The delta-delta between control and knockout is −0.03 h (p = 0.966). CrebA: For ZT15, the unpaired mean differences are −2.08 h between ZT15 control and NP control (p = 0.0) and −2.58 h between ZT15 knockout and NP knockout (p = 0.0). The delta-delta between control and knockout is −0.50 h (p = 0.489). For ZT21, the unpaired mean differences are 1.10 h between ZT21 control and NP control (p = 0.0002) and 1.44 h between ZT21 knockout and NP knockout (p = 0.0). The delta-delta between control and knockout is 0.34 h (p = 0.439). CrebB-CrebA: For ZT15, the unpaired mean differences are −2.50 h between ZT15 control and NP control (p = 0.0) and −2.89 h between ZT15 knockout and NP knockout (p = 0.0). The delta-delta between control and knockout is −0.39 h (p = 0.598). For ZT21, the unpaired mean differences are 1.29 h between ZT21 control and NP control (p = 0.0) and 1.50 h between ZT21 knockout and NP knockout (p = 0.0002). The delta-delta between control and knockout is 0.21 h (p = 0.696).
