Table 1 Free-running periods and phases of delayed fluorescence in leaves from wild-type (WT), lhy-10, gi-13 and prr7-5 hybrid aspen plants measured under continuous light

From: The circadian clock of Populus affects physiological, transcriptional and metabolomic responses to osmotic and ionic components of salt stress

Genotype

Period (h)

Phase (h)

Rhythmic/total

WT

22.8 ± 0.5

11.6 ± 0.9

5/8

lhy-10

AR

AR

0/7

gi-13

22.3 ± 0.1

16.2 ± 0.7

3/5

prr7-5

21.2 ± 0.2**

16.9 ± 0.5

6/7

  1. Hybrid aspen leaves were grown under LD 18:6 light:dark cycles (20 µmol m−2 s−1 from equal parts blue and red light during the light period). Plants were moved to continuous light (LL) at dawn. Free-running rhythms were analysed over the interval 24–120 h after transfer to LL using the FFT-NLLS algorithm (expected period 18–30 h) on the Biodare2 platform (https://biodare2.ed.ac.uk). Only rhythmic traces with a relative amplitude error (RAE) ≤ 0.6 were included in the analysis. Significant differences between periods were determined using the Kruskal–Wallis test (P < 0.001), followed by Dunn’s post hoc test (**: P < 0.01). AR indicates that all plants were arrhythmic.
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