Fig. 4: VPA may regulate FASN mRNA and protein through inhibition of mTOR signaling.
From: Valproic acid targets IDH1 mutants through alteration of lipid metabolism
A–C Representative western blots of PS6 and FASN in an IDHWT GBM cell line HK157 (A), and IDH MT GBM cell lines HK252 (B) and BT142 (C) treated with different doses of VPA for 4 days. D Representative western blot of PS6 and FASN in MT cell line HK252 treated with VPA for 2, 4, and 24 h. E Representative western blot of PS6 and FASN in MT cell line HK252 treated with rapamycin for 4 days. F Representative western blot of PS6 and FASN in MT cell line HK252 treated with VPA, rapamycin, & the combination for 4 days. G Relative mRNA expression of FASN in MT cell line HK252 treated with VPA, rapamycin, and the combination for 4 days. H Representative western blot of PS6 and FASN in MT cell line BT142 treated with VPA, rapamycin, and the combination for 4 days. I Relative cell viability after treatment with VPA, RAPA, & combination for 1 week. 2-way ANOVA and post hoc t-test ****P value<0.0001; ***P value<0.001; Error bars ± SD.
