Fig. 5: Selective FASN inhibitor TVB-2640 inhibits cell cycle genes in MT cell lines which can be recovered by supplementation of palmitate. | npj Metabolic Health and Disease

Fig. 5: Selective FASN inhibitor TVB-2640 inhibits cell cycle genes in MT cell lines which can be recovered by supplementation of palmitate.

From: Valproic acid targets IDH1 mutants through alteration of lipid metabolism

Fig. 5

A Schematic of TVB-2640 inhibiting FASN. B–F Relative cell viability of WT cell line HK157 (B) and 217 (C) and MT cell lines HK252 (D), BT142 (E) treated with TVB-2640 in the presence and absence of palmitate for 1 week. The group means ± SD are shown in F. Non-linear regression, ****P value<0.0001. G and H Dot plot showing upregulated and downregulated GO terms for MT cell lines HK252 (B) and BT142 (C) after 4 days of treatment with TVB-2640 (1 µM). I Heat map of common genes inhibited by TVB-2640 and VPA in both HK252 and BT142. J and K Relative growth of HK252 and BT142 treated with VPA (1 mM), TVB-2640 (1 µM), and PA (50 µM) for 1 week. 2-way ANOVA, post hoc t-test, ****P value < 0.0001; **P value < 0.01; **P value < 0.05.

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