Fig. 8: FASN knockdown enhances the effect of VPA in vivo.
From: Valproic acid targets IDH1 mutants through alteration of lipid metabolism
A Representative western blot showing FASN protein expression in HK252 and BT142 after FASN knockdown with three separate shRNAs. B and C Relative cell viability of MT cell lines HK252 (B) and BT142 (C) after FASN knockdown. One-Way ANOVA, ****P value <0.0001. D Relative growth of BT142 with ctrl non-target shRNA and FASN shRNA treated with VPA and PA for 1 week. E Kaplan-Meier survival curve for mice treated with either saline or VPA (300 mg/kg) implanted with NT BT142 or FASNshRNA1 KD BT142. ***P value < 0.001; *P value < 0.05. F Schematic of the mechanism of VPA and TVB-2640 in IDH MT glioma (created with bio render).
