Fig. 6: Adoptive transfer of Mhy1485 + DEBM treated SMRT KD DCs lowers Th1 responses & immune cell infiltration in DSS-induced colitis.

A. Schematic depicting the in vivo intravenous injection strategy of DCs (D-5, D-3, and D + 1), DSS treatment (D0), day of sacrifice (D6) and table showing scoring patterns for disease severity. B Line graph showing the change in disease severity index from the day of DSS induction in different experimental mice groups, (n = 10). C. Line graph demonstrating the change in body weights from the day of DSS induction in different experimental mice groups, (n = 10). D Representative image showing colon lengths and box plots demonstrating quantification of the same in DSS-induced colitis in different experimental setups, (n = 10 mice). E Violin plots exhibiting the availability of FITC-dextran in blood serum, an indication of epithelial damage during DSS treatment, (n = 3 mice). F Representative image and box plots showing spleen weights in mg, (n = 10 mice). G. Representative colon H/E sections showing infiltration of cells at 5x and 20x magnification under varying treatment conditions. H. Scatter plots and bar graphs depict cumulative percent positive and MFI levels of IFN-γ and Tbet, respectively in CD4⁺effector T cells isolated from MLNs, (n = 5 mice). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 and ****p ≤ 0.0001. p-value has been calculated using two tailed student’s t-tests (Unpaired for DSS colitis). Error bars represent SEM.