Table 1 Antibodies for flow cytometric analysis of spleens with myeloid and T cell panels.
From: Incretin triple agonist retatrutide (LY3437943) alleviates obesity-associated cancer progression
Antibody | Source | Identifiers | Dilutions |
|---|---|---|---|
Myeloid Panel | |||
Anti-Human/Mouse CD11b redFluor 710 | Tonbo | 80-0112-U025 | 1:20 |
APC anti-mouse Ly-6C | BioLegend | 128015 | 1:40 |
Anti-Mouse F4/80 Antigen PE | Tonbo | 50-4801-U025 | 1:40 |
Anti-Mouse Ly-6G PerCP-Cyanine5.5 | Tonbo | 65-1276-U025 | 1:40 |
PE/Cyanine7 anti-mouse I-A/I-E (MHC class II) | BioLegend | 107630 | 1:40 |
FITC anti-mouse CD45 | BioLegend | 103108 | 1:40 |
T Cell Panel | |||
Anti-Mouse CD8a FITC | Tonbo | 35-0081-U025 | 1:10 |
Brilliant Violet 605 anti-mouse CD4 | BioLegend | 100548 | 1:10 |
Brilliant Violet 785 anti-mouse CD3ε | BioLegend | 100355 | 1:04 |
Brilliant Violet 421 anti-mouse CD279 (PD-1) | BioLegend | 109121 | 1:10 |
PerCP/Cyanine5.5 anti-mouse CD45 | BioLegend | 103132 | 1:20 |
