Fig. 3: Mass spectrometry imaging reveals impaired cardiac energy metabolism in Fgf23CKO mice after TAC. | npj Metabolic Health and Disease

Fig. 3: Mass spectrometry imaging reveals impaired cardiac energy metabolism in Fgf23CKO mice after TAC.

From: Deletion of cardiac fibroblast growth factor-23 beneficially impacts myocardial energy metabolism in left ventricular hypertrophy

Fig. 3: Mass spectrometry imaging reveals impaired cardiac energy metabolism in Fgf23CKO mice after TAC.

A Heatmap of annotated glycan fragments and lipids, and B Partial Least Squares Discriminant Analysis (PLS-DA) score plot of the metabolome in heart cryosections reveal clear differences between LVs from Fgf23CKO TAC mice and Fgf23fl/fl TAC mice. In contrast, LVs from Sham mice of both genotypes show comparable metabolic signatures. C Intensity distribution maps and box plots of Hex1P2, HexNac1S1, Hex1P1 and DHAP (O-18:0) show distinct differences between LVs from Fgf23CKO and Fgf23fl/fl TAC mice, whereas the abundance of glycerol-3-phosphate remains unchanged. *p < 0.05, **p < 0.01, ***p < 0.001 by one-way ANOVA test followed by Tukey’s multiple comparison test. D AMP, ADP, and ATP intensity distributions and quantification of energy charge (calculated as the ratio of the concentrations of ATP, ADP, and AMP) demonstrate a pronounced reduction of energy charge in LVs of Fgf23CKO vs. Fgf23fl/fl TAC mice. *p < 0.05 by two-tailed t test. Data in (A–D) are from 3 to 4 mice per group. Hex hexose, HexNAc N-acetylhexosamine, P phosphate, S sulfate.

Back to article page