Fig. 5: Spatial transcriptomic analysis in the TAC-induced heart failure model in Fgf23CKO and Fgf23fl/fl mice.
A Spatial cluster mapping in heart cryosections shows two clearly distinct regions: Cluster 0 (red) and Cluster 1 (turquoise). B UMAP analysis of gene expression further confirms this separation, with Cluster 1 cells from both genotypes grouping closely in the upper part of the plot, while Cluster 0 cells form a separate group in the lower part. C The dot plot highlights the top 12 genes that are most differentially expressed across clusters and genotypes, illustrating key drivers of the differences between them. D Spatial expression maps for heavy chain-7 (Myh7), ANP (Nppa), and syndecan-4 (Sdc4) show distinct gene expression patterns across heart sections from Fgf23CKO (KO) and Fgf23fl/fl (Flox) mice, reflecting both cluster-specific and genotype-specific differences. Color scale indicates log-normalized gene expression per spot (Seurat LogNormalize). Blue shows lower expression, white around the average, and red higher expression.
