Fig. 5: Intravascular imaging and ex vivo perfusion setup.

a 3.7 F NIRF-IVUS imaging catheter, pullback module and acquisition system; Adapted from5,28. b Excised human coronary artery in 3D-printed holder that facilitates sectioning to allow for co-registration of imaging data and histological images. c Ex vivo ICG perfusion of human coronary arteries fixed in a holder for intravascular NIRF-IVUS imaging. ICG indocyanine green, IVUS intravascular ultrasound, NIRF near-infrared fluorescence, PMT photomultiplier tube, PZT piezoelectric element, US ultrasound; Some computer assisted design models are courtesy of Thorlabs (www.thorlabs.com).