Fig. 1: Experimental configuration and COMSOL multiphysics simulations for nanoaperture optical trapping.
From: Energy landscape of conformational changes for a single unmodified protein
a Simplified microscope diagram. Laser passes through a linear polarizer and half-waveplate to control polarisation orientation and a beam expander to fill the objective. A 100 × 1.25 NA oil immersion objective is incident on the sample and transmitted light is collected by a 10 × 0.25 NA objective and recorded on an avalanche photodiode (APD). Inset: Schematic of microwell used for trapping solution and SEM and camera image of a DNH used in experiments. Camera image is under focused to highlight the oval shape of the DNH and represent the focus state during trapping. b Electric field and (c) thermal distribution simulated by COMSOL Multiphysics for a DNH in aqueous using incident 980 nm laser with 1 mW power and beam diameter of 1 μm.
