Fig. 4: Markov chain simulations of conformational changes.

a Markov chain representation of conformations and associated probabilities and transition matrix. b Time series signal of conformational changes at 310 K, raw data in light red, identified states in dark red. c Markov chain simulation of trapping signal using values obtained from energy landscapes. d Average residence time of the N state over a range of temperatures measured from experiments and simulations. e Average residence time of the F state over a range of temperatures measured from experiments and simulations. f Average residence time of the E state over a range of temperatures measured from experiments and simulations. g Comparison of thermodynamic equilibrium constant for the N ↔ F pathway. Values obtained via energy landscape (EL) and residence times (RT). h Comparison of thermodynamic equilibrium constant for the F ↔ E pathway. Values obtained via energy landscape (EL) and residence times (RT).