Fig. 1: Analysis of the brain using the RT-QuICR (a,b) and sdRT-QuIC (c-f) assays.
From: A same day α-synuclein RT-QuIC seed amplification assay for synucleinopathy biospecimens
a–d End-point dilution analyses of single DLB (a, c) and PD (b, d) specimens. ThT fluorescence traces show means ± SD of quadruplicate reactions at the designated dilutions given with respect to the original tissue mass rather than homogenates thereof. Non-synucleinopathy (NS) brain at 10−4 dilution was included as a negative control, with the traces shown in (a, c) also applying to the plots in (b, d). e 1/TTT and f ThT maxima were obtained using 10−4 DLB (n = 5), PD (n = 4), and NS (n = 5) brain dilutions. Data points represent means of quadruplicate reactions from individual cases, and bars represent the mean ± SD of the means from all cases of that type. The same brain specimens were used in these RT-QuICR and sdRT-QuIC assays. Statistical significance is denoted as *p < 0.05, **p < 0.01, ***p < 0.001.
