Fig. 1: Schematic of a salivary Simoa assay.
From: Ultrasensitive detection of six sepsis-associated proteins in neonatal saliva
a Saliva is collected from an infant via a modified syringe and a low wall suction (collection device not pictured). For a detailed protocol description, see “Saliva acquisition” in the Methods. b Simoa consists of the formation of sandwich immunocomplexes on micron-sized beads. Substrate is added to activate a fluorescent reaction. c Each microwell array has the space to confine a single bead within each well, enabling single molecule counting. d Measurements are performed by the Quanterix HD-X Analyzer, with the output being the average number of enzymes per bead (AEB). Figure created, in part, in BioRender.com. Rolando, J. (2025) BioRender.com/u36z800.
