Table 2 Spike and recovery of Simoa assays

From: Ultrasensitive detection of six sepsis-associated proteins in neonatal saliva

Biomarker

Spike 1 concentration (pg/ml)

Mean % recovery in saliva for Spike 1

Spike 2 concentration (pg/ml)

Mean % recovery in saliva for Spike 2

Spike 3 concentration (pg/ml)

Mean % recovery in saliva for Spike 3

CXCL6

50

88

100

91

300

91

CCL20

10

95

30

113

100

105

SAA1

100

96

150

96

300

97

CXCL12

20

72

100

96

200

99

LBP

400

118

2000

86

4000

117

Resistin

20

115

100

83

200

73

  1. Each spike concentration was chosen from the upper, lower, and center ranges of the assay calibration curve. The calculated percent value is the mean of three samples. Each sample was measured in duplicate.