Fig. 2: Titration of tau-seeding activity in tissue homogenates of different brain areas from AD and non-AD. | npj Biosensing

Fig. 2: Titration of tau-seeding activity in tissue homogenates of different brain areas from AD and non-AD.

From: Regional variations in seeding activity, phosphorylation, and protein levels of tau in Alzheimer’s disease brain

Fig. 2: Titration of tau-seeding activity in tissue homogenates of different brain areas from AD and non-AD.The alternative text for this image may have been generated using AI.

a Brain homogenates from inferior-temporal gyrus (ITG, light pink), middle-temporal gyrus (MTG, light green), superior-temporal gyrus (STG, light blue) and cerebellum (CER, dark pink) of AD (n = 9) and non-AD (gray lines, n = 5), were subjected to RT-QuIC assay of tau-seeding activity at different concentrations of brain homogenates from 10−4 to 10−9 in the presence of the tau τ306/K19CFh substrates. b Comparison of end-point tau ThT fluorescence of different brain areas at the brain homogenate dilution of 10−6. *p < 0.05; **p < 0.01. c Comparison of lag times of tau RT-QuIC assay of brain homogenates at 10−6 from different areas. d Quantitative analysis of tau-seeding activity by RT-QuIC end-point dilution analysis of tissue homogenates of different brain areas of AD cases (n = 9) and non-AD controls (n = 5). The Spearman–Kärber analyses were used to determine the seeding dose (SD50) that is shown as log SD50/mg brain tissue.

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