Fig. 4: Characterization of dual-chamber SP-PCR.
From: Fast and sensitive multiplexed diagnostic system enabled by real-time solid-phase PCR assay
A Real image of the solid-phase PCR for a single target (Covid-19 virus) in C1. The immobilized FAM shows a stable and strong signal through the PCR cycle increment, while the signals of the target spot (Covid-19) gradually increase with the increment of the PCR cycles. B Real-time signal reading for the array spots for Covid-19 virus with copy numbers of 10k, 1k, and non-template target (NTC). The line represents the average of the 3 measurements of the 3 spots of the array. Annealing and denaturing times are 20 s and 5 s, respectively. C The standard curve for PCR efficiency calculation for the SP-PCR with a single target (Covid-19 virus). D Influence of annealing time (hybridization) in C1 on PCR performance. The inset of graph (D) shows the real-time fluorescence with PCR cycles. The error bars represent the standard deviation calculated from 3 independent experiments in (B–D).
