Fig. 1: Optimization of rHSA expression in N. benthamiana.

A Western blot analysis of rHSA accumulation at 2, 4-, 6-, 8-, and 10-dpi. B Western blot analysis of rHSA expression at different Agrobacterium OD600 values (0.2, 0.4, 0.6, and 0.8). C Western blot analysis of rHSA expression in plants of different ages (21-, 28-, and 35-dpg). Data were analyzed using ImageJ for densitometric quantification. Data are presented as mean ± SD (n = 3). Statistical significance was determined using one-way ANOVA (p < 0.05).