Fig. 2: Purification of HSA from Nicotiana benthamiana using Ni-NTA affinity chromatography. | npj Science of Plants

Fig. 2: Purification of HSA from Nicotiana benthamiana using Ni-NTA affinity chromatography.

From: Sustainable production of functional human serum albumin in Nicotiana benthamiana: a green biomanufacturing approach

Fig. 2

A SDS-PAGE analysis of crude extract and purified HSA, showing a single band for purified HSA at approximately 70 kDa. B Western blot analysis confirmed a band at approximately 70 kDa in both the crude extract and purified HSA, consistent with the SDS-PAGE results. The blot was probed with a 1:5,000 dilution of anti-His antibody. C: crude extract; P: purified HSA.

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