Fig. 5: Spheroid response to chemotherapy.
From: A 3D spheroid model for assessing nanocarrier-based drug delivery to solid tumors
A Incucyte® images of BxPC-3:hPSC and PANC-1:hPSC spheroids after treatment with a range of SN-38 concentrations for 72 hours. Scale bars represent 500 µm. B Dose responses of 2D PANC-1 and hPSC monocultures and PANC-1:hPSC spheroids to SN-38. C Dose responses of 2D BxPC-3 and hPSC monocultures and BxPC-3:hPSC spheroids to SN-38. Viability was quantified using CellTiter Glo® and data were compared using two-way ANOVA. Statistically non-significant differences were not labeled. D Evolution of PANC-1:hPSC spheroid diameter over time following treatment with SN-38 calculated from Incucyte® images. E Dose response of BxPC-3:hPSC spheroids to free SN-38 and SN-38 encapsulated in PluPDA NCs (SN-38@PluPDA) quantified by CellTiter Glo®. Data were compared using two-way ANOVA.
