Fig. 6: Modulating the culture environment reverses PTX therapy response in MDA-MB-231Br spheroids. | npj Biomedical Innovations

Fig. 6: Modulating the culture environment reverses PTX therapy response in MDA-MB-231Br spheroids.

From: An in vitro hydrogel-based model to study dormancy associated drug resistance in metastatic breast cancer spheroids

Fig. 6: Modulating the culture environment reverses PTX therapy response in MDA-MB-231Br spheroids.

Representative bright field images of spheroids post transfer at day 5 and at day 8 with and without treatment for A HA hydrogel to suspension and E HA hydrogel to HA hydrogel. Scale bar = 200 µm. Fold change in area upon 72 h drug treatment for spheroids transferred from B HA hydrogel to suspension and F HA hydrogel to HA hydrogel. Representative fluorescent images of EdU staining for spheroids transferred from C HA hydrogel to suspension and G HA hydrogel to HA hydrogel, blue—DAPI (nucleus), green—EdU, scale bar = 100 µm. Quantification of EdU-positive cells present in spheroids transferred from D HA hydrogel to suspension and H HA hydrogel to HA hydrogel. N ≥ 6 replicates for each condition. * Indicates statistical significance (p < 0.05) from Student’s t-test. Data presented as mean ± standard error of the mean.

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