Fig. 3: Cryo-EM structure of cytochrome bcMtb-like bound to JNJ-2901.
From: Structural and mechanistic study of a novel inhibitor analogue of M. tuberculosis cytochrome bc1:aa3
a Cryo-EM map M. smegmatis cytochrome bcMtb-like bound to JNJ-2901. Subcomplex III (bc1) is shown in light and dark green, subcomplex-IV (aa3) is shown in light and dark blue. PSRAF1 is shown in brown, and the tail of SOD in yellow. b Structure of M. smegmatis cytochrome bcMtb-like shown in cartoon representation. The red square marks the location of the JNJ-2901 binding site in one of the two copies of subcomplex III. c Close-up of JNJ-2901 binding site. QcrB is shown in teal, JNJ-2901 in magenta, and cryo-EM map is shown in gray mesh. Underlined residues (His348 & His368) are from the QcrA subunit, all other residues are from the QcrB subunit. d Schematic representation of the interactions between cytochrome bc and JNJ-2901. Hydrophobic interactions are indicated by striated half circles and hydrogen bonds by dashed black lines. Residues mutated to make cytochrome bcMtb-like are marked by a star. e Superimposition of three cytochrome bc inhibitors: JNJ-2901 (magenta), Q203 (gray, PDB:7E1W) and TB47 (sand, PDB:7E1X).
