Table 1 List of reagents
From: SARM1 base-exchange inhibitors induce SARM1 activation and neurodegeneration at low doses
Component | Supplier | Catalog number |
|---|---|---|
Compound 7 (RO-7529) | Prepared in our own laboratories | N/A |
NB-3 | Prepared in our own laboratories | N/A |
Ex. 27 | Prepared in our own laboratories | N/A |
DSRM-3716 | Sigma-Aldrich | BL3H1F1C7445-250MG |
EV-99 | Prepared in our own laboratories | N/A |
FK-866 | MedChem Express | HY-50876 |
Fingolimod (FTY-720) | Cayman Chemical | 10006292 |
Vacor | Mcule | MCULE-9179018016 |
UD1 mAb 47:3 | Uman Diagnostics | 27016 |
UD3 biotin-labeled mAb 2:1 | Uman Diagnostics | 27018 |
SULFO-TAG labeled streptavidin | Meso Scale Discovery | R32AD |
MSD GOLD Read Buffer B | Meso Scale Discovery | R60AM |
Bovine NfL | Progen | 62208 |
Human iPSC-derived motor neurons kit, 01279 | Fujifilm Cellular Dynamics, Inc. | R1049 |
Laminin | Sigma Aldrich | L2020 - 1MG |
Dulbecco’s phosphate-buffered saline (DPBS) no calcium, no magnesium | Thermo Fisher, Gibco | 14190-094 |
DAPT | Sigma Aldrich | D5942-5MG |
Dimethyl Sulfoxide (DMSO) | Sigma Aldrich | D2650-100ML |
NfL ELISA kit | Uman Diagnostics | 20-8002 |
HEPES pH 7.0 | Thermo Fisher | J62688 |
EDTA pH 8.0 | Sigma Aldrich | 03690 |
Sodium Chloride | Invitrogen | AM9759 |
Tween20 | Sigma | P9416 |
β-Nicotinamide adenine dinucleotide hydrate (NAD) | Sigma | N7004 |
β-Nicotinamide mononucleotide (NMN) | Sigma | N3501 |
DMSO | Sigma | D2438 |
Formic Acid | Fluka | 56302 |
Recombinant Human SARM1 protein (Tagged) | Abcam | ab271737 |
CellTiter-Glo® 2.0 Reagent | Promega | G9242 |
DMEM high-glucose medium | Gibco | 11965092 |
Fetal bovine serum (FBS) | Gibco | A3160502 |
Penicillin-streptomycin | Gibco | 15140122 |
