Fig. 2: CYS treatment resulted in a significant reduction of superoxide radical formation. | npj Drug Discovery

Fig. 2: CYS treatment resulted in a significant reduction of superoxide radical formation.

From: Cystobactamid off-target profiling reveals favorable safety, superoxide reduction, and SCARB1 inhibition in eukaryotes

Fig. 2: CYS treatment resulted in a significant reduction of superoxide radical formation.

a Superoxide formation in U-2 OS cells (indicated by MitoSOX-red fluorescence (pseudocolor red), Hoechst-stained nuclei (pseudocolor blue)) (scale bars are set to 200 µm). b Cells showed significantly reduced basal levels of superoxide, when treated with CN-861-2 and Cysto-180. For CN-DM-861, some precipitation was observed and cells showed no ROS induction at the maximum soluble concentration. c Co-treatment with CYS suppresses menadione-induced ROS formation. Mean values with standard deviation are shown. Statistical significance was analyzed by ordinary one-way ANOVA with multiple comparison to the control group (A) and multiple unpaired two-stage step-up t test (B) (n = 6) (ns non-significant; *p < 0.05; ****p < 0.0001).

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