Fig. 5: Cytotoxic effect of the cecropins on vertebrate cells.

A Hemolytic activity of cecropins against horse blood erythrocytes at a concentration of 200 µM. The bar chart displays hemolytic activity for each cecropin after 1 h of incubation with erythrocytes. The data were normalized to the hemolytic activity of Triton X-100 as 100% of activity and to that of the solvent DMSO in 1:5 dilution as 0%. B The left bar chart represents Madin-Darby canine kidney cells (MDCKII) (blue), and the right bar chart represents human-derived airway epithelial cells (Calu-3) (purple). The bars show cell viability after 48 h of treatment with the cecropins. All experiments were carried out in triplicate, and the data is displayed as means with standard deviation. Charts were created using GraphPad Prism 10.0.2.