Abstract
Objectives:
Currently, effective therapeutic strategy for spinal cord injury (SCI) is not clinically available. To establish a better method that may help repair the injured spinal cord, sodium hyaluronate-ciliary neurotrophic factor (CNTF) gelatinous particles were generated.
Methods:
A segment of spinal cord tissue was excised to form a 2.5-mm-long cavity at thoracic level in an adult rat, and sodium hyaluronate-CNTF gelatinous particles were implanted into the lesion cavity. The recovery of the injured spinal cord was evaluated by immunohistochemistry, nerve tracing, electrophysiological test and Basso–Beattie–Bresnahan locomotor rating scale.
Results:
Open-field locomotion of the sodium hyaluronate-CNTF rats was significantly enhanced up to 12 weeks postoperation. Together with the evidence of enhanced cortical motor evoked potentials and cortical somatosensory evoked potentials in the sodium hyaluronate-CNTF group, these findings suggested a powerful functional recovery component. Immunohistochemical analyses suggested that the functional recovery might be attributable partly to an increase in axonal regrowth as well as in replenishment of β-tubulin-III–positive neuron-like cells.
Conclusion:
Sodium hyaluronate-CNTF gelatinous particles may provide an effective method for treating SCI.
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References
Schwab ME . Repairing the injured spinal cord. Science 2002; 295: 1029–1031.
Neumann S, Woolf CJ . Regeneration of dorsal column fibers into and beyond the lesion site following adult spinal cord injury. Neuron 1999; 23: 83–91.
Bareyre FM . Neuronal repair and replacement in spinal cord injury. J Neurol Sci 2008; 265: 63–72.
Guo JS, Zeng YS, Li HB, Huang WL, Liu RY, Li XB et al. Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury. Spinal Cord 2007; 45: 15–24.
Bregman BS, Coumans JV, Dai HN, Kuhn PL, Lynskey J, McAtee M et al. Transplants and neurotrophic factors increase regeneration and recovery of function after spinal cord injury. Prog Brain Res 2002; 137: 257–273.
Ip NY, Yancopoulos GD . The neurotrophins and CNTF: two families of collaborative neurotrophic factors. Annu Rev Neurosci 1996; 19: 491–515.
Ishii K, Nakamura M, Dai H, Finn TP, Okano H, Toyama Y et al. Neutralization of ciliary neurotrophic factor reduces astrocyte production from transplanted neural stem cells and promotes regeneration of corticospinal tract fibers in spinal cord injury. J Neurosci Res 2006; 84: 1669–1681.
Paxinos G, Watson C . The rat brain in stereotaxic coordinates. In: Paxinos G, Watson C (eds). Coronal Section, 2nd edn. Academic Press: California, USA, 1986, pp 91–99.
Basso DM, Beattie MS, Bresnahan JC . A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma 1995; 12: 1–21.
Stokols S, Tuszynski MH . Freeze-dried agarose scaffolds with uniaxial channels stimulate and guide linear axonal growth following spinal cord injury. Biomaterials 2006; 27: 443–451.
Tysseling-Mattiace VM, Sahni V, Niece KL, Birch D, Czeisler C, Fehlings MG et al. Self-assembling nanofibers inhibit glial scar formation and promote axon elongation after spinal cord injury. J Neurosci 2008; 28: 3814–3823.
Teng YD, Lavik EB, Qu X, Park KI, Ourednik J, Zurakowski D et al. Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc Natl Acad Sci USA 2002; 99: 3024–3029.
Barnabé-Heider F, Göritz C, Sabelström H, Takebayashi H, Pfrieger FW, Meletis K et al. Origin of new glial cells in intact and injured adult spinal cord. Cell Stem Cell 2010; 7: 470–482.
Ke Y, Chi L, Xu R, Luo C, Gozal D, Liu R . Early response of endogenous adult neural progenitor cells to acute spinal cord injury in mice. Stem Cells 2006; 24: 1011–1019.
Danilov AI, Covacu R, Moe MC, Langmoen IA, Johansson CB, Olsson T et al. Neurogenesis in the adult spinal cord in an experimental model of multiple sclerosis. Eur J Neurosci 2006; 23: 394–400.
Imaizumi T, Lankford KL, Kocsis JD . Transplantation of olfactory ensheathing cells or Schwann cells restores rapid and secure conduction across the transected spinal cord. Brain Res 2000; 854: 70–78.
Koyanagi I, Tator CH . The effects of cortical stimulation, anesthesia and recording site on somatosensory evoked potentials in the rat. Electroencephalogr Clin Neurophysiol 1996; 101: 534–542.
Jou IM . Effects of core body temperature on changes in spinal somatosensory-evoked potential in acute spinal cord compression injury: an experimental study in the rat. Spine 2000; 25: 1878–1885.
Barnabé-Heider F, Frisén J . Stem cells for spinal cord repair. Cell Stem Cell 2008; 3: 16–24.
Acknowledgements
This work was supported by the State Key Program of National Natural Science Foundation of China (Grants nos. 31130022, 31271037 and 31320103903), the National Science and Technology Pillar Program of China (Grant no. 2012BAI17B04), the National 863 Project (Grant no. 2012AA020506), the National Ministry of Education Special Fund for Excellent Doctoral Dissertation (Grant no. 201356), the Special Funds for Excellent Doctoral Dissertation of Beijing, China (Grant no. 20111000601) and the Key Project of Department of Science and Technology of Beijing, China (Grant no. D090800046609004).
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Wang, N., Zhang, S., Zhang, A. et al. Sodium hyaluronate-CNTF gelatinous particles promote axonal growth, neurogenesis and functional recovery after spinal cord injury. Spinal Cord 52, 517–523 (2014). https://doi.org/10.1038/sc.2014.54
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DOI: https://doi.org/10.1038/sc.2014.54
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