Figure 1: The schematic illustrates the design and testing process used in developing the dataset.
From: Dataset of eye disease-related proteins analyzed using the unfolding mutation screen
The first step was designing the program. The program was written in Python, R and Bash. The purpose of the program was to calculate the unfolding propensity for each missense mutation in a proteins structure. Following, an effective display of the data was pivotal. Next the program was validates using data for 16 proteins obtained from the Protherm database. After the validation human models of proteins related to inherited eye disease were built using homology model. The quality of the structures was tested using the internal control program. Finally UMS was applied to these proteins to produce the mutation matrix, standard and clustered heat maps, and the patterned structure.
