Figure 1: Work flow.
From: Single-cell RNA sequencing of mouse brain and lung vascular and vessel-associated cell types
(a) Flowchart of the procedure of generating the single cell dataset. (b) Whole adult mouse brains from the indicated mouse reporter lines were mechanically and enzymatically digested, and single cells were isolated by FACS, cDNA libraries prepared and sequenced. (c) Single cell transcriptomes were clustered by BackSPIN. The black bar-plot shows Actb expression (sequence counts) in the 38 clusters (0–37) generated at split-level 6: these clusters were given a preliminary cell class assignment (black-colored bars) using canonical cell type-specific markers. (d) After cluster consolidation, a final annotation was provided for individual cells. (e) The average expression (+/− standard error) of each cluster is summarized. Gene-by-gene expression figures are available at http://betsholtzlab.org/VascularSingleCells/database.html. Figure c–d overlap with Extended Data Figure 1b–c,i in our related publication (ref. 4).
