Figure 2
From: Structural and functional insights into sorting nexin 5/6 interaction with bacterial effector IncE
The IncE:PX interface. (a) Zoom-in view of interactions between PX5 and IncE from the crystal structure. For clarity, IncE is shown in ribbon representation. Possible interacting (except hydrogen bonds) residues are shown in stick representation. (b) Glutathione S-transferase (GST) pull down of GST-IncE109–132 WT and mutants with purified PX5 protein. (c) SPR steady state analysis of GST-IncE109–132 WT and two mutants binding to immobilized PX5. (d) MBP pull down of MBP-IncE109–132 with GST-PX5 WT and mutants. Before running SDS-PAGE, the samples are digested with excess of tobacco etch virus to allow visualization of bound PX5 protein. (e) Immunoprecipitation of green fluorescent protein (GFP)-SNX6 using GFP antibody with purified GST-IncE WT and two mutants.
