Figure 4
Prediction of unconventional splicing of bZIP60 mRNA.
a, Stem-loop structures observed in bZIP60, XBP1 and HAC1 mRNA. The conserved nucleotides essential for splicing of HAC1 and XBP1 mRNAs are indicated in boxes. The corresponding nucleotides conserved in bZIP60 mRNA are also indicated in boxes. Triangles indicate the splicing sites observed in HAC1 and XBP1 mRNAs, as well as the sites predicted in bZIP60 mRNA. b, Nucleotide sequence alignment of unspliced and spliced bZIP60, HAC1 and XBP1 cDNA around the splicing sites. Asterisks and triangles indicate the conserved nucleotides and the splicing sites, respectively. c, Nucleotide and deduced amino acid sequences around the splicing sites of bZIP60 cDNA. The splicing sites are indicated by triangles. The amino acid sequence corresponding to the putative TMD is indicated by an unshaded box and the sequence translated after splicing was indicated by a shaded box as ORF2. Complete sequences of bZIP60u and bZIP60s are presented in Supplementary Fig. S5 online. d, Schematic representations of bZIP60u, bZIP60s and bZIP60ΔC. The transcriptional activation domain is indicated by AD.
