Figure 3

Molecular basis for JH recognition by JHBP.

Interactions of JHBP with the epoxy moiety (a) and the ester moiety (b) of JH II in the crystalline state. Residues involved in the recognition are shown as stick models and JH II as a ball-and-stick model. The transparent molecular surfaces of aromatic residues are also shown. Hydrogen bonds and stacking interactions are indicated by light blue and orange dotted lines, respectively. (c) Schematic diagram of the JH recognition mechanism by JHBP. JH III binding affinities of the single-mutant JHBPs relative to the wild-type protein are shown in the parentheses. Polar and non-polar interactions are indicated by arrows and circular arcs, respectively. The interactions occurring behind JH III are shown as dashed lines. Mutants with substitutions of residues involved in non-polar interactions alone and in both polar and non-polar interactions are shown in red and blue, respectively.