Figure 3
From: Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1
Silver stain of hVN1R1.
Four distinct peaks were observed on the 280 nm UV trace through a size exclusion column (SEC). Samples corresponding to the peaks were pooled and run on a gel. The first peak (lane 1) primarily corresponded to higher molecular weight aggregates. The second peak (lane 2) contained dimerized receptor. The third peak (lane 3) contained a monomeric version of hVN1R1. This form of the receptor constituted over 60% of the protein recovered after SEC and was present in all of the samples. The fourth peak contained the elution peptide and is not shown.
