Figure 6

Inferring protein function from predicted PPIs.

Biological process may be inferred based on the annotations of predicted interaction partners. (A) Predicted PPIs for 8 novel human proteins (yellow and green nodes) against known dsDNA break repair proteins (blue nodes). Red edges represent novel predicted interactions and the green edge represents a previously reported interaction. (B) Yeast deletion mutant strains for genes homolog to human novel genes above are subjected to plasmid repair assay. The number of colonies formed after strain transformation with linearized plasmid is normalized to that of intact plasmid and related to the wild type (red bar) strain (set at 100%). YKU70 and RSC2 are known players in non-homologous repair of double stranded DNA breaks and are used as positive controls (pink bars). Yellow bars represent strains with statistically significant (P-value < 0.05) reduction efficiency in plasmid repair. The strains that did not show reduction in plasmid repair efficiency are represented by green bars. Each experiment was repeated at least four times. Error bars in this figure represent the standard deviation between experiments.