Figure 5
From: Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration
CRN2, CK2 and F-actin co-localize in the front of lamellipodia.
Pop10 cells over-expressing GFP-tagged CRN2 were fixed and CK2α was immunolabeled with primary antibody 1AD9 followed by Alexa-633 labeled secondary antibody; F-actin and nuclei were visualized by TRITC-phalloidin and DAPI, respectively. CRN2 and CK2 co-localize in the peri-nuclear region (arrowheads); CRN2, CK2 and F-actin co-localize in the front of lamellipodia (arrows). Double-arrowheads, F-actin fibers decorated by CRN2.
