Figure 4

¹⁹F/¹H MRI and Electron Microscopy of the ¹⁹F-rich Particles within the Cerebellum of an EAE Mouse Brain.

(A) Horizontal slice of the previous SJL/J mouse-brain using 2D-FLASH imaging: TR = 473 ms, TE = 13 ms, 22 slices, in-plane resolution 73 μm², NEX = 16 (scan time = 25 min). The black outlined box depicts the slice selection for the ¹⁹F acquisition. (B) Median sagittal slice of the same mouse brain. (C) ¹⁹F image of the 3 mm central slice using 2D FLASH TR = 15 ms, TE = 3.3 ms, 1 3 mm slice, in-plane resolution (400 × 400) μm², NEX = 2048 (scan time = 15 min) and (D) an overlay of (C) (in gray) and (D) (in red). (E) to (H) Histological slices of the extracted cerebellum. (E) Differential interference contrast image of a cerebellar lesion (toluidine blue staining) which shows small round and white nanoparticles engulfed into macrophage like cell structures (red arrows). (F) to (H) high resolution electron microscopy images of adjacent regions.