Figure 6

Visualization of Inflammatory Cells in different CNS and Lymphatic Regions using In Vivo ¹⁹F/ ¹H MRI.

EAE was induced in SJL/J mice and 170 nm ¹⁹F-rich and DiI-labeled nanoparticles (5 μmoles ¹⁹F) were administered daily, starting from day 5 following immunization. (A) Two different sagittal slices of a 3D Turbo RARE-scan show the kinetics of inflammatory cell migration ( ¹⁹F MRI) over a period of 6 days in different CNS and lymphatic regions (¹H MRI). In vivo ¹H sequence (as above) and ¹⁹F MRI sequence: TR = 800 ms, TE = 6 ms, FOV = (40 × 16 × 16) mm³, Matrix = 100 × 40 × 40, zero fill acceleration 2, RARE Factor = 40, NEX = 256 (scan time = 68 min). (B) Distribution of inflammatory cells (specifically CD3⁺, CD4⁺, F4-80⁺ and Mac-2⁺cells) in EAE tissue that gave a strong ¹⁹F signal in MRI. Shown in coronal sections of the cerebellum are specific cell populations (green) and their corresponding uptake of ¹⁹F-DiI nanoparticles (red). Arrows indicate colocalization (yellow) of signal from specific immune cell markers (green) and ¹⁹F-DiI nanoparticles (red).