Figure 1
From: Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.
Strategy for targeted disruption of the NOP1 locus and analysis of homologous recombination events.
(a) Structure of pJHY-TMp1 and pJHY-CMp1. Only the structure between the left and right borders (LB and RB, respectively) of each vector is shown. DT-A, Diphtheria toxin gene; hpt, hygromycin phosphotransferase gene; ΔEn, 3′ part of the maize En element; gus, β-glucuronidase gene. (b) Schematic representation of the genomic structure of the wild-type NOP1 gene region. (c) Structure of pKI406. (d) Structure of the NOP1 locus disrupted by HR. The exons and introns of the NOP1 gene are indicated by blue and open boxes, respectively. The green bar represents the NOP1 flanking region carried by pKI406. Primers are indicated by triangles. Recognition sites of EcoRI and HindIII are indicated by vertical lines with the letters E and H, respectively.
