Table 1 Numbers of Hmr plants obtained by positive/negative selection and targeted events at the NOP1 locus

From: Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.

Experiment

Vector

Number of Hmr plants

Plants with the nop1 phenotype

A1)

pJHY-CMp1

1424

0

B1)

pJHY-CMp1

1168

0

C1)

pJHY-CMp1

1312

0

D1)

pJHY-TMp1

7

0

E1)

pJHY-TMp1

6

0

F1)

pJHY-TMp1

22

0

G2)

pKI406

276

4

H2)

pKI406

172

4

I2)

pKI406

195

7

J2)

pKI406

220

3

K2)

pKI406

67

2

  1. 1)Each transformation was performed using a sporangium and transformation efficiency per sporangium was estimated.
  2. 2)Transformation was performed using 4 sporangia.