Table 2 Evaluation of targeting efficiency for NOP1 locus using vectors containing various homologous arms
From: Homologous recombination-mediated gene targeting in the liverwort Marchantia polymorpha L.
Experiment | Vector | Number of Hmr plants1) | Number of plants with nop1 phenotype | Targeting efficiency |
|---|---|---|---|---|
A | pKI406 | 163 | 7 | Â |
B | pKI406 | 210 | 5 | 3.2% (12/373) |
C | pKI406-1kb | 231 | 0 | Â |
D | pKI406-1kb | 273 | 0 | Â |
E | pKI406-1kb | 175 | 0 | <0.015% (0/679) |
F | pKI406-2kb | 372 | 2 | Â |
G | pKI406-2kb | 194 | 2 | Â |
H | pKI406-2kb | 250 | 2 | 0.7% (6/816) |
E | pKI406-d1kb | 333 | 4 | Â |
F | pKI406-d1kb | 319 | 4 | Â |
G | pKI406-d1kb | 316 | 2 | 1.0% (10/968) |
H | pKI406-d3kb | 408 | 0 | Â |
E | pKI406-d3kb | 397 | 1 | Â |
F | pKI406-d3kb | 203 | 0 | 0.1% (1/1008) |
