Figure 4
From: Ubiquinone-quantum dot bioconjugates for in vitro and intracellular complex I sensing
Complex I sensing in vitro.
(a–c), Time-dependent fluorescence changes upon the interaction of Q2NS (a), Q5NS (b) and Q10NS (c) functionalised CdSe/ZnS QDs with complex I (4.9 U) in the presence of 10 mM NADH. The insets illustrate the increase of fluorescence (ΔF) as a function of incubation time. (d) Calibration curve corresponding to the fluorescence analysis of variable concentrations of complex I of QnNS-QDs in the presence of NADH. (e) Absorption spectra changes observed upon addition of complex I to a deaerated PBS solution of Q5NS-functionalised CdSe/ZnS QDs in the presence of 10 mM NADH. All measurements were performed in a 0.2 M deaerated PBS solution of pH 8.0.
