Figure 2
From: Inorganic coatings for optimized non-viral transfection of stem cells
Systematic change of mineral coating crystallinity and its effect on mineral stability.
(a) FTIR spectra of CaP mineral coatings. The peaks are associated with the PO43− (…) and CO32− (− −) groups. (b) The effect of mSBF solution characteristics on resulting mineral crystallinity index, which was calculated using FTIR analysis (Supplementary Fig. S1). (c) Ca/P ratio of mineral coatings. * indicates significant difference when compared with Ca/P ratio of CaP mineral coating grown in 2 × mSBF with 4.2 mM of CO32− (red bar at far left) by Student's t-test with p < 0.05. Data represents mean ± SD (n = 3). (d) Dissolution of mineral coatings formed in 12 different mSBF solutions. The change of Ca2+ amount in cell culture medium was measured at different time points. Data represents mean ± SD (n = 4). (e) The effect of relative crystallinity on mineral stability. The released Ca2+ amount after 2 days incubation in cell culture medium is plotted in terms of crystallinity index of mineral coatings. Data represents mean ± SD (n = 4).
