Figure 3 | Scientific Reports

Figure 3

From: Analysis of SUMOylated proteins using SUMO-traps

Figure 3

Multiple SUMO substrates can be captured by SUBE-l.

SUMOylated (A) PML, (B) IκBα, (C) p53 and (D) PTEN were SUMOylated in vitro with SUMO-1 (S1) or SUMO-2/-3 (S2/3) to generate modified proteins (input). Control reactions (-) do not contain SUMO activating enzyme. SUBE-l captured proteins are present in the bound fraction. Analysis by Western-blot using the indicated antibodies. The arrow shows unmodified proteins. * non specific signal.

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