Figure 4
Capture of SUMOylated PML and p53 using SUBE-l.
NB4 cells (A) or MCF7 (B) were pre-treated with MG-132 before 1 h of ATO treatment. (C) SUMO and PML can be co-localized in the nuclear bodies (NB) of NB4 cells under the same conditions than (A or B). SUMOylated p53 can be captured by SUBE-l (D). HEK-293 were untreated or treated with IFN-α or infected with VSV. Input, flow-through (FT) and bound fractions were analysed with anti-SUMO-2/-3, PML or p53 antibodies. GAPDH (glyceraldehyde 3-phosphate dehydrogenase) was used as loading control.
