Figure 4

Average rest intensity by age groups as interpreted by different activity monitoring models.
Bar graphs depicting daily averages of rest intensity over an 11-day period as calculated using different models for light periods or dark (lights-off) periods and full diet or sugar diet flies respectively. Each triad of charts compares rest intensity (left to right) across three age periods, 15–25 days, 50–60 days and 80–90 days. Each bar denotes the average rest intensity recorded by a specific activity model. Rest intensity is calculated as number of hours of rest/12-hour period (light or dark). This is then averaged over an 11-day period for each age group. Dark orange and light orange bars depict results from enclosure bisection simulation models, X-axis bisection and Y-axis bisection respectively. Five blue-shaded bars from navy to cyan (left to right) depict the results from distance displacement simulation models, 20 mm, 15 mm, 10 mm, 5 mm and 2 mm models. Red bars denote the rest levels calculated by the BMS. (A) Rest intensity in full diet A. ludens females during daily 12-hour light periods across ages and models. (B) Full diet average rest intensity during daily 12-hour dark periods. (C) and (D) Average rest intensity in sugar-only diet A. ludens females for daily 12-hour light periods and dark periods respectively. n = 16 for each treatment.