Figure 4
From: Zebrafish crypt neurons project to a single, identified mediodorsal glomerulus
DiI injection in mdg2 glomerulus labels crypt neurons, in contrast to injection in neighboring glomeruli.
Localized injections of DiI were placed at the approximate position of mdg2 and dlg1 glomerulus and verified after tracing ((e), (i) and (g), (k), respectively). False color is used to distinguish mdg2 injections (red) and dlg1 injections (blue) in all panels. Results shown are from one animal each for mdg2 and dlg1 position. (a), (c), brightfield micrographs show olfactory epithelium, olfactory bulb and anterior telencephalon. (e), (g), fluorescent images of the same area show the injection sites after the tracing period. (i), (k), overlay of brightfield and fluorescent images above shows the position of injection sites. Red oval in k) represents the site of injection into mdg2. Second (mdg2 injection) and fourth (dlg1 injection) column show tracing results in cryostat sections of the olfactory epithelium. (b), (d), several cells are labeled within a section; (f), (h), enlargements from another (f) or the same section (h). (J) nine representative cells backtraced from mgd2 position from several different sections are shown at high magnification; (l). likewise for dlg1 position. Note the clear difference in morphology between cells backtraced from mdg2 and the adjacent dlg1 position. Scale bars, b,d (80 μm) and (f,h) (10 μm) respectively.
