Figure 2

Apigenin impairs miRNA maturation.
(a), Cells were treated with the appropriate substances for 24 h and the indicated proteins were blotted. Representative results from three independent experiments using Huh7 cells are shown. Full-length blot images are available in Supplementary Figure 5a. (b), The expression levels of mature miRNAs and miRNA precursors were determined by qRT-PCR using Huh7 cells with or without apigenin treatment for 24 h. Data represent the means ± s.d. from three independent experiments. *, p < 0.05 (t-test) compared with the control (DMSO only) treatment.