Figure 1
From: Epigenetic silencing of microRNA-203 is required for EMT and cancer stem cell properties
Low miR-203 expression is associated with EMT, claudin-low breast cancer and stem-like cells and the promoter of miR-203 is methylated in HMLE-Twist.
(a) A microarray was performed to gauge changes in microRNA expression due to EMT induction in HMLE cells by Snail, Twist, Goosecoid or TGF-β1 overexpression. MicroRNAs with greater than 2-fold change in any at least two of the indicated cell lines are plotted. (b) DNA methylation levels at CpG islands were determined by DREAM analysis of HMLE and HMLE-Twist cells. MiRNAs for which microarray-derived expression data is available are color-coded to indicate the direction of their expression change after an EMT. (c/d) Relative levels of miR-200c and miR-203 were determined in cell lines induced to undergo EMT (c) and in established breast cancer cell lines (d) by qRT-PCR. (e) HMLE cells were FACS-sorted for CD44 and CD24. Relative levels of miR-200c and miR-203 were measured by qRT-PCR in the two subpopulations.
