Figure 3
From: CSF1R mutations in hereditary diffuse leukoencephalopathy with spheroids are loss of function
Csf1r expression in mCsf1r Ba/F3 cells.
(A) Flow cytometry analysis of Csf1r expression on naive and wild-type mCsf1r Ba/F3 cells cultured in IL-3 or rhCSF1, respectively. (B) Left: Expression of Csf1r on naïve cells cultured in IL-3 and on mutant cells capable of growth in rhCSF1; K584E, R814P and V659I/T661A. Right: Expression of Csf1r on naïve and K614R mutant cells cultured in IL-3. (C) Expression of Csf1r on mCsf1r mutant cells cultured in IL-3 alone or both IL-3 and rhCSF1 compared to naïve cells. All cells were gated on live cells as determined by negative staining for propidium iodide.
