Figure 3
From: MafK positively regulates NF-κB activity by enhancing CBP-mediated p65 acetylation
MafK regulates NF-κB -dependent gene expression.
(a) Regulation of NF-κB activity after MafK knockdown or overexpression. The NF-κB-response-element-Luc reporter was cotransfected with MafK siRNA or MafK plasmids into HepG2 cells. After 24 h, cells were treated with LPS and incubated for 24 h, then lysed for the luciferase assay. Expression of firefly luciferase reporter was normalized to Renilla luciferase expression. (b) Cells were transfected with MafK or control siRNA for 48 h. Cells were treated with LPS (1 μg/mL) followed by measurement of NF-κB-dependent gene expression in lysates after 1, 3 and 5 h. (c) Cells were transfected with MafK or control plasmids for 48 h. Cells were treated with LPS and NF-κB-dependent gene expression was measured after 1, 3 and 5 h.
